Triple Sugar Iron Agar (In accordance with IP 1996)
Details
Recommended for identification of Gram-negative enteric bacilli on the basis of dextrose, lactose and sucrose fermentation and hydrogen sulphide production in accordance with IP, Triple Sugar Iron Agar should be used in parallel with Urea Agar or Broth to distinguish between Salmonella and Proteus species, The reactions can be interpreted as follows: Alkaline slant / acid butt- only glucose fermented, Acid slant / acid butt- dextrose and sucrose fermented or dextrose and lactose fermented or all the three sugars, dextrose,lactose and sucrose fermented, Bubbles or cracks present-gas production Black precipitate present-H2S gas production
