Thermo Fisher Scientific CD16/CD32 Monoclonal Antibody (93), NovaFluor Yellow 690, eBioscience

Thermo Fisher Scientific CD16/CD32 Monoclonal Antibody (Clone 93), NovaFluor Yellow 690

Applications

• Flow Cytometry (tested)

Tested Dilution: 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Red 685 / 700 / 710 / 755
• PE / PE-Cyanine7
• PerCP-Cyanine5.5 / PerCP-eFluor 710
• Super Bright 436 / 600
• Custom conjugation available

Product Specific Information

The 93 monoclonal antibody recognizes an epitope shared by mouse CD16 and CD32.
CD16 (FcγRIII) and CD32 (FcγRII) are low-affinity IgG Fc receptors expressed by B cells, macrophages, NK cells, and neutrophils.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes (≤1.0 µg/test in 100 µL volume).
Cell concentration: 10⁵–10⁸ cells/test. Optimize antibody titration for best performance.

Important Notes:

• NovaFluor dyes are incompatible with DNA-intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
  Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  Add 5 µL per sample to antibody cocktails prior to cell addition.
• For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).
• NovaFluor conjugates use Phiton™ technology for engineered fluorescence with minimized spillover and spread.
• Excitation: 552 nm; Emission: 690 nm; Laser: 561 nm (Yellow).
• Non-specific staining observed in SJL mice B cells; not observed in BALB/c or C57BL/6 mice.

Target Information

CD16: 50–65 kDa surface molecule; exists in transmembrane and PI-linked forms. Expressed by NK cells, some T cells, and granulocytes. Mediates ADCC via FcγRIII.

CD32: 40 kDa glycoprotein; low-affinity receptor for IgG (FcγRII).
Functions include endocytosis, secretion activation, cytotoxicity, and immunomodulation.
Expressed by B cells, monocytes, granulocytes, and platelets.
Involved in immune complex phagocytosis and antibody production regulation.
Certain CD32 variants may increase susceptibility to systemic lupus erythematosus (SLE).

For Research Use Only. Not for diagnostic procedures or resale without authorization.

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