
Thermo Fisher Scientific Syrian Hamster IgG Isotype Control, Brilliant Violet 480, eBioscience
시리안 햄스터 IgG 아이소타입 컨트롤 항체로, Brilliant Violet 480 형광체가 결합된 Flow Cytometry용 음성 대조 항체입니다. 405 nm 레이저 장비에서 사용 가능하며, 비특이적 결합을 구분하는 데 유용합니다. 4°C에서 보관하며, 동결 금지.
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Applications
Tested Dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (Flow) | Assay-dependent |
| Control (Ctrl) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Syrian hamster / IgG |
| Class | Polyclonal |
| Type | Isotype Control |
| Conjugate | Brilliant Violet™ 480 |
| Excitation/Emission Max | 437 / 478 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping conditions | Wet ice |
| RRID | AB_3090513 |
Product Specific Information
This hamster IgG fraction was purified by affinity chromatography from normal Golden Syrian Hamster serum.
Applications Reported: Suitable for use in flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Use at the same concentration as the experimental antibody.
Brilliant Violet™ 480 (BV480) emits at 479 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Ensure your instrument can detect this fluorochrome.
When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.
Brilliant Violet™ 480 (BV480) is not compatible with methanol-based fixation.
Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser.
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Target Information
Isotype control antibodies help distinguish non-specific background signal from specific antibody signal, as they have no relevant specificity to a target antigen.
They are useful in applications such as:
- Flow cytometry
- Chromatin immunoprecipitation (ChIP)
- Immunohistochemistry
- Gel shift assays
Isotype controls should match the species and isotype of the primary antibody to accurately determine specific staining levels.
When using directly labeled primary antibodies, the isotype control should be conjugated with the same label.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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