Thermo Fisher Scientific Mouse IgG2b kappa Isotype Control (eBMG2b), Brilliant Violet 480, eBioscience
Brilliant Violet 480 형광체로 결합된 Mouse IgG2b kappa 동형 대조 항체로, 유세포분석용으로 최적화됨. 비특이적 결합 평가에 사용되며, 405 nm 레이저 시스템에 적합. 4°C 암소 보관, 메탄올 고정 불가.
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Applications
- Flow Cytometry (Flow): Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBMG2b |
| Conjugate | Brilliant Violet™ 480 |
| Excitation/Emission Max | 437 / 478 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925643 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 647, 660, 700
- APC, APC-eFluor 780, Biotin
- Brilliant UV 395, 496, 563, 615, 661, 737, 805
- Brilliant Violet 421, 605, 650, 711, 786
- eFluor 450, 506, 570, 615, 660
- FITC, Functional Grade, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation available
Product Specific Information
The monoclonal mouse IgG2b kappa is useful as an isotype control immunoglobulin for flow cytometric analysis.
This eBMG2b antibody has been tested by flow cytometric analysis of normal human peripheral blood and mouse splenocytes. Use at the same concentration as the experimental antibody.
Brilliant Violet™ 480 (BV480) emits at 479 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Ensure instrument compatibility with this fluorochrome.
When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
Note: BV480 is not compatible with methanol-based fixation.
Excitation: 440 nm
Emission: 479 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Target Information
Mouse IgG2b is a subtype of immunoglobulin G antibody produced by B cells in response to antigens. It is the third most abundant IgG subtype in serum and plays a key role in the adaptive immune response.
It is composed of two heavy and two light chains and is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity.
Mouse IgG2b also regulates immune responses, including T cell activation and B cell inhibition.
It is widely used in research and diagnostic applications such as ELISA, Western blotting, and immunohistochemistry, and in therapeutic research including immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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