Thermo Fisher Scientific BUBR1 Monoclonal Antibody (8G1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:5,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Flow Cytometry (Flow)

1 µg/1 million cells

-

Immunoprecipitation (IP)

1:10-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

8G1

Immunogen

Residues 1-350 of human BUBR1, tagged at the N-terminus with pMAL. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BUBR1,uniProtId:O60566-1,ncbiNodeId:9606,antigenRange:1-350,antigenLength:1050,antigenImageFileName:MA1-16577_BUBR1_O60566-1_House_mouse.svg,antigenImageFileNamePDP:MA1-16577_BUBR1_O60566-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• HRP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568472

Product Specific Information

Suggested positive control: Hela whole cell extract, antigen standard for BUB1B (transient overexpression lysate).

Target Information

BUBR1 is a member of the Ser/Thr protein kinase family. It is a component of the mitotic checkpoint that delays anaphase until all chromosome are properly attached to the mitotic spindle. Compromised spindle checkpoints are thought to play an important role in genetic instability that predisposes cells to malignant transformations. Human cells contain two related checkpoint protein kinases, evolved from the BUB1 gene; hBUBR1 and hBUB1. They are important components of the spindle checkpoint Both kinases monitor mitotic kinetochore-microtubule interactions. hBUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. It has been identified as a novel binding partner of BCSG1 (Breast Cancer-Specific Gene 1), which has been suggested to accelerate the progression of breast cancer by compromising the mitotic checkpoint control through inactivation of BUBR1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.