
Thermo Fisher Scientific BUBR1 Monoclonal Antibody (8G1)
BUBR1 단백질을 인식하는 Thermo Fisher Scientific의 단일클론 항체(8G1)로, 인간·마우스·랫트 반응성을 가집니다. Western blot, ICC/IF, Flow cytometry, IP 등 다양한 응용에 사용 가능하며, Protein G 정제 및 PBS 버퍼에 보관됩니다. 연구용 전용 시약입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1 µg/1 million cells |
| Immunoprecipitation (IP) | 1:10–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8G1 |
| Immunogen | Residues 1–350 of human BUBR1, tagged at the N-terminus with pMAL |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_568472 |
Additional Formats
Product Specific Information
Suggested positive control: HeLa whole cell extract, antigen standard for BUB1B (transient overexpression lysate).
Target Information
BUBR1 is a member of the Ser/Thr protein kinase family and a key component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. Defects in this checkpoint contribute to genetic instability and cancer progression. Human cells express two related checkpoint kinases, hBUBR1 and hBUB1, derived from the BUB1 gene. Both monitor kinetochore–microtubule interactions during mitosis. hBUBR1 prevents premature anaphase entry and has been detected in various human cancers. It interacts with BCSG1, which may promote breast cancer progression by impairing BUBR1 function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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