Thermo Fisher Scientific FANCD2 Monoclonal Antibody (FI-17)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:10-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-Dependent

-

ChIP assay (ChIP)

1:10-1:500

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

FI-17

Immunogen

FANCD2 fusion protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_568533

Product Specific Information

By Western blot, this antibody recognizes a band at ~155 and ~162 kDa (post-translationally modified form).

Suggested positive control: Hela whole cell extract.

Target Information

FANCD2 (Fanconi anemia group D2 protein) is required for maintenance of chromosomal stability. It promotes accurate and efficient pairing of homologs during meiosis. FANCD2 is involved in the repair of DNA double-stranded breaks, both by homologous recombination and single-stranded annealing. It is required for the targeting/stabilization of BLM to non-centromeric abnormal structures induced by replicative stress. FANCD2 promotes BRCA2/FANCD1 loading onto damaged chromatin. Defects in the FANCD2 gene result in Fanconi anemia complementation group D2. This disorder affects all bone marrow elements resulting in anemia, leukopenia, and thrombopenia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.