Thermo Fisher Scientific LIMK1/LIMK2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino acids 500-550 of human LIMK1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LIMK1/LIMK2,uniProtId:P53667-1,ncbiNodeId:9606,antigenRange:500-550,antigenLength:647,antigenImageFileName:PA5-36917_LIMK1LIMK2_P53667-1_Rabbit.svg,antigenImageFileNamePDP:PA5-36917_LIMK1LIMK2_P53667-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2553813

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 72 kDa.

Purity is >95% by SDS-PAGE.

Target Information

Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, including LIMK-1 and LIMK-2, has been identified. LIMK-1 has been shown to regulate the stabilization of F-Actin structures and cofilin activity, indicating that LIMK-1 plays a role in a signaling pathway involved in the regulation of cell motility and morphogenesis. LIMK-1 inhibits neuronal differentiation of PC12 cells, and is thought to act by interfering with events downstream of MAPK activation. Expression patterns of LIMK-1 and LIMK-2 suggest that these proteins may have different functions during development. A truncated form of LIMK-2 has been identified in adult testis that is thought to arise from an alternative initiation exon.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.