Thermo Fisher Scientific CD40 Monoclonal Antibody (OTI4D12)

Applications

Flow Cytometry (Flow)

• Tested Dilution: 1:100
• Publications: [References not listed]

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional round of desalting (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The CD40 antigen is a single-chain glycoprotein belonging to the tumor necrosis factor/nerve growth factor superfamily, showing significant homology to CD30.
It is expressed on all B cells except plasma cells, and also on some epithelial cells, carcinomas, and lymphoid dendritic cells.
CD40 plays a crucial role in immune and inflammatory responses, including:

• T cell-dependent immunoglobulin class switching
• Memory B cell development
• Germinal center formation

AKNA transcription factor regulates CD40 expression, important for cell interactions.
Adaptor protein TNFR2 interacts with CD40, mediating signal transduction.
CD40-CD40L interaction is necessary for amyloid-beta-induced microglial activation, implicated in Alzheimer’s disease pathogenesis.
Two alternatively spliced transcript variants encoding distinct isoforms have been reported.
Diseases associated with CD40 dysfunction include Type 3 Hyper-IgM immunodeficiency and CD40 ligand deficiency.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.