Thermo Fisher Scientific UBA52 Monoclonal Antibody (OTI4F2), TrueMAB
UBA52 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot과 IHC(P)에서 검증됨. 합성 펩타이드 면역원으로 제작되었으며, 동결건조 형태로 제공. PBS 완충액에 트레할로스를 포함하고, 보존제 무첨가. 연구용으로 단백질 분해 및 리보솜 단백질 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4F2 |
| Immunogen | Synthetic peptide corresponding to residues near C-terminus of human UBA52 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Ubiquitin is a highly conserved protein involved in targeting cellular proteins for degradation by the 26S proteasome.
The UBA52 gene encodes a fusion protein consisting of ubiquitin at the N-terminus and ribosomal protein S27a at the C-terminus.
Post-translational processing generates free ubiquitin monomer and ribosomal protein S27a, a component of the 40S ribosomal subunit.
S27a contains C4-type zinc finger domains and is located in the cytoplasm.
Pseudogenes derived from this gene exist in the genome.
Similar fusion proteins include ribosomal protein L40 (with ubiquitin) and ribosomal protein S30 (with ubiquitin-like fubi).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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