Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), TrueMAB

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Flow Cytometry (Flow)

1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

UCHT1

Immunogen

Human infant thymocytes and lymphocytes from a patient with Sezary disease if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD3,uniProtId:P07766-1,ncbiNodeId:9606,antigenRange:1-207,antigenLength:207,antigenImageFileName:CF807198_CD3_P07766-1_House_mouse.svg,antigenImageFileNamePDP:CF807198_CD3_P07766-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:CD3,uniProtId:P04234-1,ncbiNodeId:9606,antigenRange:1-171,antigenLength:171,antigenImageFileName:CF807198_CD3_P04234-1_House_mouse.svg,antigenImageFileNamePDP:CF807198_CD3_P04234-1_House_mouse_PDP.jpeg,sortOrder:2},{targetFamily:CD3,uniProtId:P09693-1,ncbiNodeId:9606,antigenRange:1-182,antigenLength:182,antigenImageFileName:CF807198_CD3_P09693-1_House_mouse.svg,antigenImageFileNamePDP:CF807198_CD3_P09693-1_House_mouse_PDP.jpeg,sortOrder:5},{targetFamily:CD3,uniProtId:P20963-1,ncbiNodeId:9606,antigenRange:1-164,antigenLength:164,antigenImageFileName:CF807198_CD3_P20963-1_House_mouse.svg,antigenImageFileNamePDP:CF807198_CD3_P20963-1_House_mouse_PDP.jpeg,sortOrder:6}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.