Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), PerCP-eFluor 710, eBioscience
마우스 CD31(PECAM-1) 인식 단클론 항체로 PerCP-eFluor 710 결합 형식. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 488 nm 레이저로 여기되어 710 nm에서 방출. 내피세포 및 백혈구 표지에 활용 가능. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | View 90 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 390 |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation/Emission Max | 482/708 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1834430 |
Additional Available Formats
- Unconjugated
- APC
- Biotin
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 711
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 685 / 710 / 725 / 755
- NovaFluor Yellow 610
- PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- Super Bright 436 / 600 / 645
- Request custom conjugation
Product Specific Information
Description:
The 390 monoclonal antibody reacts with mouse CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed by endothelial cells and at low levels on leukocytes and platelets. CD31 is involved in cell adhesion, signaling, and cell–cell/matrix interactions.
Applications Reported:
Validated for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometry using mouse spleen and bone marrow cells. Recommended ≤0.125 µg/test (100 µL final volume). Optimize concentration empirically.
Fluorophore Information:
PerCP-eFluor® 710 emits at 710 nm and is excited with the 488 nm blue laser. Recommended filter: 710/50 bandpass (695/40 acceptable). Ensure instrument compatibility.
Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:
- IC Fixation Buffer (Product #00-8222-49)
- 1-step Fix/Lyse Solution (Product #00-5333-54)
Optical Properties:
- Excitation: 488 nm
- Emission: 710 nm
- Laser: Blue laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD31 (PECAM-1):
CD31 is an inhibitory coreceptor regulating T and B cell signaling through ITIM motifs, providing docking sites for phosphatases upon phosphorylation. Expressed in the vascular system, primarily at endothelial junctions. The N-terminal Ig-like domain mediates homophilic binding, crucial for cell–cell interactions.
Biological Roles:
- Integrin-mediated adhesion
- Transendothelial migration
- Angiogenesis and apoptosis
- Negative regulation of immunoreceptor signaling
- Autoimmunity and macrophage phagocytosis
- IgE-mediated anaphylaxis and thrombosis
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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