Thermo Fisher Scientific FOXP3 Polyclonal Antibody
FOXP3 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal antibody로, WB, IHC, ICC, IP 등 다양한 응용에 적합합니다. Human과 Mouse 반응성이 있으며, 고순도 항원 친화 크로마토그래피로 정제된 액상 형태입니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific FOXP3 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse FOXP3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris Glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792379 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%).
- Positive Control: mouse brain.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals and essential for normal immune homeostasis.
FOXP3 is 381 amino acids long, expressed at high levels in CD25+CD4+ regulatory T cells, low levels in CD4+/CD25− cells, and absent in CD4−/CD8+ T cells.
Defects in FOXP3 cause the scurfy phenotype in mice and IPEX syndrome in humans (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome).
In human breast and colon cancer cells, FOXP3 expression is regulated by p53 in response to DNA damage.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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