Thermo Fisher Scientific CDK9 Polyclonal Antibody
CDK9 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat에 반응하며, 고순도의 항원 친화 크로마토그래피로 정제됨. 세포주 HL-60, NIH-3T3에서 양성 대조로 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CDK9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792381 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (86%), Xenopus laevis (85%), Pig (98%), Chicken (87%), Rhesus Monkey (100%), Bovine (98%).
- Positive Control: HL-60, NIH-3T3.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
CDK9 is a serine/threonine kinase which, when complexed with Cyclin T1, forms an active holoenzyme complex that regulates the eukaryotic cell cycle. CDK9 is coexpressed and copurified with Cyclin T1 and regulates cytokine-inducible transcription networks by facilitating promoter recognition of target transcription factors. It promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. The CDK9/cyclin-K complex also has kinase activity toward the CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 causes cardiac myocyte enlargement leading to cardiac hypertrophy and predisposition to heart failure.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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