Thermo Fisher Scientific LCK Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Immunoprecipitation (IP)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Keep as concentrated solution.
• Predicted reactivity: Human (88%), Mouse (85%), Rat (84%), Pig (84%), Sheep (85%), Bovine (85%).
• Positive Control: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

LCK is a member of the Src-family tyrosine kinases essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates ITAM motifs in zeta subunits, creating binding sites for SH2 domains of tyrosine kinase ZAP70, which is activated to form signaling platforms via LAT phosphorylation.

Most LCK localizes to the plasma membrane, with a fraction associated with the Golgi apparatus contributing to Raf activation under weak TCR stimulation. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2/SH3 domains mediating interactions with phosphotyrosine-containing and proline-rich motifs.

LCK also regulates apoptosis induced by various stimuli but not by death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternative splice variants encoding different isoforms have been identified.

⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm. For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.