Thermo Fisher Scientific Human GPD2 Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

Amino acids Ala43-Tyr600 containing an N-terminal His-tag

Tag

His-tag

Class

Recombinant

Type

Protein

Purity

>95%, by SDS-PAGE under reducing conditions

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.095 mg/mL

Purification

purified

Storage buffer

20mM tris HCl, pH 8, with 50% glycerol, 1mM EDTA

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

This product contains purified recombinant human mitochondiral Glycerol-3-PO4 Dehydrogenase protein with an N-terminal hexahistidine tag.

This product may be used as a positive control in many immunological assays.

Target Information

Mitochondrial glycerophosphate dehydrogenase (EC 1. 1. 99. 5), or GPD2, is located on the outer surface of the inner mitochondrial membrane and catalyzes the unidirectional conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Together with a cytosolic NAD-linked GPD (GPD1; MIM 138420), GPD2 forms the glycerol phosphate shuttle, which uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, resulting in the reoxidation of NADH formed during glycolysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.