Thermo Fisher Scientific GGA3 Polyclonal Antibody
Rabbit polyclonal antibody against human GGA3 protein. Validated for WB, ELISA, and IP applications. High specificity and affinity purified. Reacts with human and chimpanzee; potential cross-reactivity with macaque and orangutan. Supplied as liquid, 1 ...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| ELISA | 1:10,000–1:50,000 |
| Immunoprecipitation (IP) | 1:500–1:3,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chimpanzee, Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity-purified antibody prepared from whole rabbit serum immunized with a synthetic peptide corresponding to an internal region near amino acids 400–425 of Human GGA3. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at –20°C prior to opening. Aliquot contents and freeze at –20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against human GGA3 protein. BLAST analysis suggests reactivity with human and chimpanzee GGA3 based on 100% sequence homology. Cross-reactivity may occur with macaque (93% homology) and orangutan (87% homology). The immunizing sequence is present on both the long (isoform 1) and short (isoform 2) transcript variants of this protein.
Target Information
The GGA3 gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding family. These coat proteins regulate trafficking of proteins between the trans-Golgi network and lysosome. They contain an amino-terminal VHS domain for sorting mannose 6-phosphate receptors and a carboxy-terminal region homologous to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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