Thermo Fisher Scientific eNOS Polyclonal Antibody
eNOS 단백질을 인식하는 Rabbit Polyclonal Antibody로 다양한 종(Human, Mouse, Pig, Rabbit, Rat)에 반응합니다. WB, IHC, ICC, Flow Cytometry, ELISA 등 여러 응용에 사용 가능하며, Protein A로 정제된 고순도 항체입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Pig, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human NOS-3 (amino acids 1105–1202) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission, and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidation of L-arginine to produce L-citrulline and NO.
Three isoforms of NOS have been cloned:
- nNOS (type I): neuronal NOS
- iNOS (type II): inducible NOS
- eNOS (type III): endothelial cell NOS
All NOS isoforms contain calmodulin, NADPH, FAD, and FMN binding domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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