Thermo Fisher Scientific beta-2 Microglobulin Recombinant Rabbit Monoclonal Antibody (4H5L6)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody is predicted to react with non-human primate, mouse, and rat based on sequence homology.
Intact IgG appears on a non-reducing gel as a ~150 kDa band and, upon reduction, generates a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to the large rabbit immune repertoire

Target Information

Beta-2-microglobulin (B2M) is a 12–14 kDa protein that forms a subunit of the MHC class I complex. It is associated with HLA Class I antigens and expressed on various cell types including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial, and epithelial cells, but absent on erythrocytes.

It is also found in free form in plasma. The amino acid sequence of B2M is homologous to the constant regions of immunoglobulin heavy and light chains, with a predominantly beta-pleated sheet structure.

Functions and biological relevance:

• Involved in antigen processing and presentation
• Plays a role in TCR signaling and downstream signaling in naïve CD8+ cells
• Encoded by the B2M gene on chromosome 15
• Mutations can cause hypercatabolic hypoproteinemia
• Levels of B2M are used to assess renal function and as a prognostic marker for HIV infection

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.