Thermo Fisher Scientific S1PR3 (EDG3) (extracellular) Polyclonal Antibody, Atto 488

Applications

• Flow Cytometry (Flow): 5–10 µg/5×10⁵ cells

Product Specifications

Product Specific Information

• Reconstitution: Add 50 µL of double distilled water (DDW), depending on sample size.
• The antibody ships as a lyophilized powder at room temperature. Upon arrival, store at -20°C.
• Reconstituted solution can be stored at 4°C for up to 1 week. For longer storage, aliquot and keep at -20°C.
• Avoid multiple freeze/thaw cycles.
• Centrifuge all antibody preparations before use (10,000 × g, 5 min).

Target Information

EDG-3 (S1PR3) belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-3 is sphingosine-1-phosphate.
There are 8 known members of the EDG receptor family, involved in growth-related effects such as:

• Induction of cellular proliferation
• Alterations in differentiation and survival
• Suppression of apoptosis

These receptors also mediate cytoskeletal-dependent cellular responses such as contraction, secretion, adhesion, and chemotaxis.
EDG receptors are developmentally regulated, differ in tissue distribution, and couple to multiple G proteins to signal through Ras, MAP kinase, Rho, phospholipase C, and protein tyrosine kinases.
EDG-3 is expressed in cardiovascular, leukocyte-containing, and other tissues.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.