Thermo Fisher Scientific S1PR3 (EDG3) (extracellular) Polyclonal Antibody, Atto 488

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5-10 µg/5x10^5 cells

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)DYVGKLAGRLRD, corresponding to amino acid residues 23-34 of mouse S1PR3, Extracellular, N-terminus if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EDG3,uniProtId:Q9Z0U9-1,ncbiNodeId:10090,antigenRange:23-34,antigenLength:378,antigenImageFileName:ASR-013-AG-50UL_EDG3_Q9Z0U9-1_Rabbit.svg,antigenImageFileNamePDP:ASR-013-AG-50UL_EDG3_Q9Z0U9-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Atto™ 488 Atto™ 488 Atto™ 488

Excitation/Emission Max

501/523 nm

Form

Lyophilized

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

EDG-3 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-3 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-3 is expressed in cardiovascular, leukocyte-containing and other tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.