Thermo Fisher Scientific CD27 Monoclonal Antibody (OTI3F1)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily, which includes CD40 and CD30. Members of this family regulate cell proliferation and death.
CD27 and its ligand CD70 are primarily expressed on lymphocytes. High CD27 expression depends on antigen receptor ligation, while CD70 expression requires co-stimulatory or pro-inflammatory signals.
CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells, and a subset of B cells. Upon T cell activation via the TCR-CD3 complex, CD27 expression increases and a soluble 28–32 kDa form (sCD27) is released, detectable in plasma, urine, or spinal fluid.
Soluble CD27 serves as a prognostic marker for acute and chronic B cell malignancies.
RgpA, a cysteine proteinase, can degrade CD27 and counteract CD27/CD70-mediated T cell activation. CD27-binding protein (SIVA) is a proapoptotic protein that binds CD27 and may mediate apoptosis through this receptor.
Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.