Thermo Fisher Scientific MCF2 Monoclonal Antibody (OTI3E4), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:500

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

MCF2 is a guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. It promotes the conversion of Rho family GTPases from GDP-bound to GTP-bound forms.

• Isoform 1: No activity toward RHOA, RAC1, or CDC42
• Isoform 2: Decreased GEF activity toward CDC42
• Isoform 3: Weak but significant activity toward RAC1 and CDC42
• Isoform 4: Significant activity toward RHOA and CDC42
• Truncated DBL oncogene: Active toward RHOA, RAC1, and CDC42

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.