Thermo Fisher Scientific MCF2 Monoclonal Antibody (OTI3E4), TrueMAB
MCF2 단백질을 인식하는 마우스 단클론 항체로, Western blot에 최적화되어 있습니다. 인간 유래 단백질에 반응하며 동결건조 형태로 제공됩니다. 재구성 후 약 1 mg/mL 농도로 사용 가능하며, 연구용으로만 사용됩니다.
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Applications
- Western Blot (WB): Tested dilution 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3E4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 872–1001 of human MCF2 produced in E. coli. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MCF2 is a guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. It promotes the conversion of Rho family GTPases from GDP-bound to GTP-bound forms.
- Isoform 1: No activity toward RHOA, RAC1, or CDC42
- Isoform 2: Decreased GEF activity toward CDC42
- Isoform 3: Weak but significant activity toward RAC1 and CDC42
- Isoform 4: Significant activity toward RHOA and CDC42
- Truncated DBL oncogene: Active toward RHOA, RAC1, and CDC42
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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