Thermo Fisher Scientific MEK2 N-Term Monoclonal Antibody (19G10.F1.E2), FITC
MEK2 단백질의 N-말단을 인식하는 FITC 결합 단클론 항체로, 인간, 생쥐, 랫트 시료에 반응합니다. Western blot 및 ELISA에 적합하며, 높은 특이성과 재현성을 제공합니다. 단백질 A로 정제되었으며, 형광 검출에 용이합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 19G10.F1.E2 |
| Immunogen | Synthetic peptide corresponding to amino acid residues near the N-terminus of MEK2 conjugated to KLH |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Available Formats
- Unconjugated
- Biotin
- DyLight 680
- DyLight 800
- HRP
- Custom conjugation available upon request
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid repeated freeze/thaw cycles. Centrifuge if not clear after standing at room temperature. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Cross-reactivity is expected with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK1 isoform.
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase involved in mitogen growth factor signal transduction. MEK2 activates ERK1/2 and MAPK enzymes by phosphorylating residues within the TEY motif of the activation loop. MEK1/2 are activated by dual phosphorylation on serine 218 and 222, while threonine 292 of MEK1 is phosphorylated by ERK2 as a negative feedback mechanism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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