
Thermo Fisher Scientific CASC3 Polyclonal Antibody
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CASC3 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, IP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태. Human에 반응하며 Mouse, Rat, Bovine에도 예측 반응함. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 225 and 275 of human cancer susceptibility candidate 3 (CASC3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, and Bovine
Target Information
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of two complexes: a 20S core and a 19S regulator.
- The 20S core consists of four rings of 28 non-identical subunits (two rings of 7 alpha subunits and two rings of 7 beta subunits).
- The 19S regulator includes a base (6 ATPase and 2 non-ATPase subunits) and a lid (up to 10 non-ATPase subunits).
- Proteasomes are abundant in eukaryotic cells and cleave peptides in an ATP/ubiquitin-dependent, non-lysosomal pathway.
- The immunoproteasome, a modified form, processes class I MHC peptides and contains the 11S regulator (PA28) instead of the 19S regulator.
- This gene encodes the gamma subunit of the 11S regulator; six gamma subunits form a homohexameric ring.
- Two transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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