Thermo Fisher Scientific Ksp-Cadherin/CDH16 Monoclonal Antibody (CDH16, 2125)
인간 CDH16(Ksp-Cadherin)을 인식하는 Mouse monoclonal antibody. Western blot, IHC(P), Peptide Array에서 검증됨. 단백질 결합 특이성이 높고, 신장 특이적 발현 연구에 적합. 단백질 A/G 정제, PBS buffer, 보존제 없음.
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- 1014-MSM5-P1ABX
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- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CDH16, 2125 |
| Immunogen | Recombinant fragment (around aa 371–507) of human CDH16 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Antibody is tested in direct ELISA.
- For immunohistochemistry (PFA-fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Cadherins are a family of Ca²⁺-dependent adhesion molecules that mediate cell-cell binding critical for structural maintenance and morphogenesis.
Cadherin-16 (Ksp-Cadherin) is a type I membrane protein expressed specifically in the kidney, localized to the basolateral membrane of renal tubular epithelial cells.
The human Ksp-cadherin gene (CDH16) maps to chromosome 16q21–proximal 16q22, while the mouse gene is localized to a syntenic region of distal chromosome 8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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