Thermo Fisher Scientific EphB4 Monoclonal Antibody (5B5)
인간 EPHB4 단백질을 인식하는 Mouse IgG1 단클론 항체. Western blot, ICC/IF, ELISA에 적합. 순수화된 재조합 EPHB4 단백질로부터 제조. 혈관 발달 및 신경계 연구에 활용 가능. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5B5 |
| Immunogen | Purified recombinant extracellular fragment of human EPHB4 fused with hIgGFc tag expressed in HEK293 cell line |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10980378 |
Product Specific Information
MA5-15506 targets EPHB4 in indirect ELISA, IF, and WB applications, showing reactivity with human samples.
The immunogen is a purified recombinant extracellular fragment of human EPHB4 fused with an hIgGFc tag expressed in HEK293 cells.
MA5-15506 detects EPHB4, which has a predicted molecular weight of approximately 108 kDa.
Target Information
EPHB4 is a receptor tyrosine kinase belonging to the ephrin receptor family. Members of the Eph family play key roles in biological processes such as nervous system development, angiogenesis, and neural synapse formation and maturation.
Ephrins are divided into ephrin-A (EFNA) and ephrin-B (EFNB) classes based on their membrane anchoring mechanisms. Eph receptors are similarly categorized based on extracellular domain sequence similarity and ligand affinity. Ephrin receptors constitute the largest subgroup of the receptor tyrosine kinase (RTK) family.
EphB4 binds to ephrin-B2 and plays an essential role in vascular development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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