Thermo Fisher Scientific EphB4 Monoclonal Antibody (5B5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:200-1:1,000

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5B5

Immunogen

Purified recombinant extracellular fragment of human EPHB4 fused with hIgGFc tag expressed in HEK293 cell line.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10980378

Product Specific Information

MA5-15506 targets EPHB4 in indirect ELISA, IF and WB applications and shows reactivity with Human samples.

The MA5-15506 immunogen is purified recombinant extracellular fragment of human EPHB4 fused with hIgGFc tag expressed in HEK293 cell line.
.

MA5-15506 detects EPHB4 which has a predicted molecular weight of approximately 108kDa.

Target Information

EPHB4 is a receptor tyrosine kinase that belongs to the ephrin receptor family. Members of the Eph family of kinases play important roles in diverse biological processes including nervous system development, angiogenesis and neural synapsis formation and maturation. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by EphB4 binds to ephrin-B2 and plays an essential role in vascular development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.