Thermo Fisher Scientific CD80 Monoclonal Antibody (2A2)

Applications

Tested Dilution

Publications

Western Blot (WB)

-

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Immunohistochemistry (IHC)

-

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Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

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Immunocytochemistry (ICC/IF)

1:200-1:1,000

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ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2A2

Immunogen

Purified recombinant fragment of CD80 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. not determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10981139

Product Specific Information

MA5-15512 targets CD80 in indirect ELISA, IF and IHC applications and shows reactivity with Human samples.

The MA5-15512 immunogen is purified recombinant fragment of CD80 expressed in E. Coli.

MA5-15512 detects CD80 which has a predicted molecular weight of approximately 55kDa.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.