Thermo Fisher Scientific Cyclophilin B Polyclonal Antibody, Alexa Fluor 488

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-10 µg/mL

-

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C(194) G K I E V E K P F A I A K E(208) of human CyPB if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cyclophilin B,uniProtId:P23284-1,ncbiNodeId:9606,antigenRange:194-208,antigenLength:216,antigenImageFileName:PA1-027A-A488_Cyclophilin_B_P23284-1_Rabbit.svg,antigenImageFileNamePDP:PA1-027A-A488_Cyclophilin_B_P23284-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2662701

Product Specific Information

PA1027AA488 detects cyclophilin B (CyPB) in mouse, rat, canine, and human tissues.

The PA1027AA488 immunogen is a synthetic peptide corresponding to residues C(194) G K I E V E K P F A I A K E(208) of human CyPB. This sequence is almost completely conserved between human, mouse, rat, bovine, and chicken. PA1027AA488 immunizing peptide (Cat. # PEP-058) is available for use in neutralization and control experiments.

Target Information

Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins which bind FK506 are termed FK506 Binding Proteins (FKBPs) and those which bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation. Thus, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIases, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.