Thermo Fisher Scientific Cyclophilin B Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 54 publications 54 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Flow Cytometry (Flow)

3-5 µg/1x10^6 cells

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Fruit fly, Guinea pig, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C(194) G K I E V E K P F A I A K E(208) of human CyPB. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cyclophilin B,uniProtId:P23284-1,ncbiNodeId:9606,antigenRange:194-208,antigenLength:216,antigenImageFileName:PA1-027A_Cyclophilin_B_P23284-1_Rabbit.svg,antigenImageFileNamePDP:PA1-027A_Cyclophilin_B_P23284-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2169138

Product Specific Information

PA1-027A detects cyclophilin B (CyPB) in mouse, rat, canine, and human tissues.

PA1-027A has been successfully used in Western blot procedures, IF/ICC and IHC (P). By Western blot, this antibody detects a ~19 kDa protein representing CyPB from rat liver extract.

The PA1-027A immunogen is a synthetic peptide corresponding to residues C(194) G K I E V E K P F A I A K E(208) of human CyPB. This sequence is almost completely conserved between human, mouse, rat, bovine, and chicken. PA1-027A immunizing peptide (Cat. # PEP-058) is available for use in neutralization and control experiments.

Target Information

Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins which bind FK506 are termed FK506 Binding Proteins (FKBPs) and those which bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation. Thus, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIases, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.