Thermo Fisher Scientific MARK1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

2.5 µg/mL

-

Product Specifications

Species Reactivity

Horse, Human, Non-human primate, Rabbit

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic 19 amino acid peptide from N-terminus of human MARK1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MARK1,uniProtId:Q9P0L2-1,ncbiNodeId:9606,antigenRange:1,antigenLength:795,antigenImageFileName:PA5-32915_MARK1_Q9P0L2-1_Rabbit.svg,antigenImageFileNamePDP:PA5-32915_MARK1_Q9P0L2-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2550376

Product Specific Information

Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Horse, Rabbit (100%); Dog, Bovine, Panda (95%); Rat, Hamster, Elephant, Platypus (89%); Mouse, Opossum, Turkey, Chicken, Lizard (84%).

For IHC(P), use heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Target Information

MARK1 (MARK) is a serine/threonine kinase which is involved in cytoskeletal organization and biogenesis. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. MARK1 is involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.