Thermo Fisher Scientific SCN1A Polyclonal Antibody

Thermo Fisher Scientific SCN1A Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody is predicted to react with bovine, frog, and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is brain tissue.

Target Information

Epithelial sodium channels are amiloride-sensitive members of the Degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels.
Members of this superfamily share structural similarity, possessing two short intracellular amino and carboxyl termini, two short membrane-spanning segments, and a large extracellular loop with a conserved cysteine-rich region.
There are three homologous isoforms of the ENaC protein: alpha, beta, and gamma.
ENaC in the kidney, lung, and colon plays an essential role in trans-epithelial sodium and fluid balance and mediates aldosterone-dependent sodium reabsorption in the distal nephron, thus regulating blood pressure.
ENaC is thought to be regulated through association with the cystic fibrosis transmembrane conductance regulator (CFTR) chloride ion channel.
Gain-of-function mutations in beta- or gamma-ENaC can cause severe arterial hypertension (Liddels syndrome), whereas loss-of-function mutations in alpha- or beta-ENaC cause pseudohypoaldosteronism (PHA-1).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 파일명: PA1-38631_SCN1A_P04774-1_Rabbit.svg, PA1-38631_SCN1A_P04774-1_Rabbit_PDP.jpeg)