Thermo Fisher Scientific CUGBP1 Recombinant Rabbit Monoclonal Antibody (ARC1866)
CUGBP1 단백질을 인식하는 토끼 유래 재조합 단클론 항체로, Western blot, ELISA, Immunoprecipitation에 적합합니다. 인간, 마우스, 랫트 시료에 반응하며, 고순도 친화 크로마토그래피 정제 및 안정한 PBS/glycerol buffer로 제공됩니다.
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1866 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1–100 of human CELF1 (Q92879) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897757 |
Product Specific Information
- Positive Samples: SH-SY5Y, Mouse brain, Rat brain
- Immunogen sequence:
MNGTLDHPDQ PDLDAIKMFV GQVPRTWSEK DLRELFEQYG AVYEINVLRD RSQNPPQSKG CCFVTFYTRK AALEAQNALH NMKVLPGMHH PIQMKPADSE
Target Information
Myotonic dystrophy (MD) is an autosomal dominant neuromuscular disease associated with a (CTG)n repeat expansion in the 3′-untranslated region of the myotonin protein kinase (Mt-PK) gene. A (CUG)n oligonucleotide triplet repeat pre-mRNA/mRNA binding protein, CUG-BP1, plays an important role in DM pathogenesis.
HeLa cell protein CUG-BP1 binds specifically to (CUG)8 oligonucleotides in vitro and represents the major (CUG)8 binding activity in normal cells. CUG-BP1 has been identified as isoforms of the heterogeneous nuclear ribonucleoprotein (hnRNP) hNab50.
CUG-BP/hNab50 is localized predominantly in the nucleus and associates with polyadenylated RNAs in vivo. RNA-binding and photocrosslinking studies demonstrate that CUG-BP/hNab50 binds to RNAs containing the Mt-PK 3′-UTR. The (CUG)n repeat region in Mt-PK mRNA serves as a binding site for CUG-BP/hNab50 in vivo, and repeat expansion leads to sequestration of this hnRNP on mutant Mt-PK transcripts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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