Thermo Fisher Scientific POLB Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

-

ELISA (ELISA)

1:50,000

-

Immunoprecipitation (IP)

0.5 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of the POLß protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:POLB,uniProtId:P06746-1,ncbiNodeId:9606,antigenRange:335,antigenLength:335,antigenImageFileName:600-401-C65_POLB_P06746-1_Rabbit.svg,antigenImageFileNamePDP:600-401-C65_POLB_P06746-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody reacts with overexpressed and endogenous POL beta protein. A BLAST analysis was used to suggest reactivity with POL beta from human, bovine, and Xenopus laevis based on a 100% homology with the immunizing sequence. Cross-reactivity with POL beta from other sources has not been determined.

Target Information

DNA polymerase beta comprises an amino-terminal 8- kDa domain and a carboxy-terminal 31- kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity. Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with the DNA-alkylating agents such as N-methyl-N`-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.