Thermo Fisher Scientific POLB Polyclonal Antibody
POLB 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, WB, ELISA, IP에 사용 가능. 고순도 친화 크로마토그래피 정제 제품이며, 인간 POLB 단백질과 반응. -20°C 보관, 연구용 전용.
- 카탈로그번호
- 600-401-C65x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific POLB Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5 µg/mL |
| ELISA | 1:50,000 |
| Immunoprecipitation (IP) | 0.5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of the POLß protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- This product is stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody reacts with overexpressed and endogenous POL beta protein.
A BLAST analysis suggests reactivity with POL beta from human, bovine, and Xenopus laevis based on 100% homology with the immunizing sequence.
Cross-reactivity with POL beta from other sources has not been determined.
Target Information
DNA polymerase beta comprises an amino-terminal 8-kDa domain and a carboxy-terminal 31-kDa domain.
The N-terminal ssDNA binding domain has deoxyribose phosphodiesterase activity, while the C-terminal domain has nucleotidyltransferase activity.
Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment with DNA-alkylating agents such as MNNG or methyl methanesulfonate up-regulates beta-pol levels.
DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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