Thermo Fisher Scientific CTCF Polyclonal Antibody
CTCF 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western Blot, IHC, ELISA에 적합합니다. Human 및 Mouse에 반응하며, 고순도 친화 크로마토그래피로 정제되었습니다. -20°C 보관 시 안정적이며 연구용으로 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| ELISA | 1:10,000–1:50,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CTCF affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of CTCF protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody reacts with endogenous CTCF protein. A BLAST analysis suggests reactivity with CTCF from human, mouse, horse, bovine, panda, rabbit, Danio, and chicken based on 100% homology with the immunizing sequence. Cross-reactivity with CTCF from other sources has not been determined.
Target Information
CTCF is a ubiquitously expressed transcriptional factor containing 11 zinc finger domains. It plays key roles in transcriptional regulation, gene silencing, chromatin insulation, and imprinting control. CTCF interacts with the CCCTC DNA motif and can associate with both HAT- and HDAC-containing complexes, influencing transcriptional activation or repression. It regulates genes such as APPB, c-myc, Pax6, and IRAK2, and is implicated in various cancers including breast, prostate, and Wilms' tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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