Thermo Fisher Scientific CNF1/CNF2 Monoclonal Antibody (JC4)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• MA1-40276 detects CNF1/CNF2 from E. coli samples.
• Successfully used in ELISA, functional assays, and Western blot applications.
• Immunogen corresponds to residues 169–191 of the N-terminus of CNF1.
• Endotoxin level: <24 EU/mg.

Target Information

Cytotoxic necrotizing factor type 1 and 2 (CNF1 & CNF2) belong to a family of bacterial toxins that target small GTP-binding Rho proteins regulating the actin cytoskeleton.
While most toxins in this family inactivate Rho, CNF1 and CNF2 activate Rho by deamidation.
CNF1 is commonly associated with E. coli strains causing extraintestinal infections in humans, particularly urinary tract infections (e.g., cystitis, pyelonephritis, prostatitis).
In CNF1-producing uropathogenic E. coli, CNF1 is chromosomally encoded and located on a pathogenicity island that also contains hemolysin and P fimbria-related genes.
Both CNF1 and the related plasmid-encoded CNF2 are monomeric cytoplasmic toxins (~115 kDa) with three functional domains: N-terminal, central, and C-terminal, the latter exhibiting catalytic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.