Thermo Fisher Scientific CNF1 Monoclonal Antibody (NG8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50

-

ELISA (ELISA)

1:50

-

Functional Assay (Functional)

1:50

-

Product Specifications

Species Reactivity

Bacteria

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

NG8

Immunogen

C-terminus of CNF1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.1% BSA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1073738

Product Specific Information

MA1-40275 detects CNF1 from E. coli samples.

MA1-40275 has been successfully used in ELISA, functional assays and Western blot applications.

The MA1-40275 immunogen is the C-terminus of CNF1.

Endotoxin <24 EU/mg.

Target Information

Cytotoxic necrotizing factor type 1 and 2 (CNF1 & CNF2) belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho; however, CNF1 and the highly related CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis and prostatitis). In CNF1-producing uropathogenic E. coli strains, CNF1 is chromosomally encoded and typically resides on a pathogenicity island that also contains hemolysin and P fimbria- related genes. Both CNF1 and the highly related, plasmid-encoded CNF2 are monomeric, cytoplasmic toxins of approximately 115 kDa. CNF1 can be structurally organized into three functional domains the N-terminal, central and the C-terminal domain. The latter exhibits the catalytic activity of the toxin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.