Thermo Fisher Scientific MCM3 Recombinant Rabbit Monoclonal Antibody (ARC0607)
MCM3 단백질을 표적하는 Recombinant Rabbit Monoclonal Antibody로, Western blot, IHC, ICC/IF, ELISA, IP 등 다양한 응용에 적합합니다. HEK293 세포에서 발현되었으며, 고순도 Affinity Chromatography 정제. 인간, 마우스, 랫트 반응성. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0607 |
| Immunogen | Synthetic peptide corresponding to amino acids 1–100 of human MCM3 (UniProt ID: P25205) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849020 |
Product Specific Information
Immunogen sequence:
MAGTVVLDDV ELREAQRDYL DFLDDEEDQG IYQSKVRELI SDNQYRLIVN VNDLRRKNEK RANRLLNNAF EELVAFQRAL KDFVASIDAT YAKQYEEFYV
Target Information
MCM3 is one of the highly conserved mini-chromosome maintenance proteins involved in the initiation of eukaryotic genome replication. The MCM2–7 hexameric complex forms a key component of the pre-replication complex (pre_RC) and participates in replication fork formation and recruitment of other DNA replication-related proteins. MCM3 interacts directly with MCM5/CDC46 and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. This acetylation inhibits DNA replication initiation and cell cycle progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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