Thermo Fisher Scientific Phospho-ATM (Ser1981) Monoclonal Antibody (10H11)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

MA1-2020 detects phospho-ATM kinase in human and mouse samples.
It has been successfully used in immunofluorescence, immunoprecipitation, and Western blot procedures.
By Western blot, this antibody detects a ~370 kDa protein representing phospho-ATM kinase in crude lysates from gamma-irradiated HeLa cells.
In immunofluorescence, MA1-2020 recognizes phospho-ATM kinase in irradiated human and mouse fibroblasts.
The immunogen is a phosphorylated synthetic peptide corresponding to residues S(1974) L A F E E S(p) Q S T T I S S(1988) of human ATM kinase protein.

Target Information

Ataxia-telangiectasia Mutated (ATM) is a protein belonging to the PI3/PI4 kinase family.
Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurological degeneration, immunologic deficiency, and increased risk of lymphoid cancer.
ATM phosphorylates proteins instead of lipids and has downstream targets such as P53, Mdm2, BRCA1, and SMC1.
ATM is essential for repairing double-stranded DNA breaks caused by ionizing radiation and other mutagens.
Its C-terminal region shares homology with catalytic domains of PI3 kinases.
ATM becomes autophosphorylated and activated upon exposure to ionizing radiation, regulating cell cycle, apoptosis, and DNA damage repair.
Studies link ATM to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.