Thermo Fisher Scientific APAF1CT Polyclonal Antibody
Apaf-1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western Blot, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. Human, Mouse, Rat, Zebrafish에 반응하며, Protein A 정제 및 1 mg/mL 농도의 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human Apaf-1 (amino acids 1201–1248) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains WD-40 domains, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, it forms an oligomeric apoptosome that activates caspase 9, triggering the apoptotic cascade. Alternative splicing produces several isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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