Thermo Fisher Scientific beta-2 Defensin Polyclonal Antibody, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human BD-2.
Anti-Human BD-2-specific antibody was purified by affinity chromatography employing an immobilized Human BD-2 matrix.

Sandwich ELISA:
To detect Human BD-2 by sandwich ELISA (100 µL/well antibody solution), use 0.5–2.0 µg/mL of this antibody.
When used with PeproTech Biotinylated Anti-Human BD-2 (500-P161GBt) as a detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Human BD-2.

Western Blot:
To detect Human BD-2 by Western Blot, use 0.1–0.2 µg/mL.
With compatible secondary reagents, detection limit for Recombinant Human BD-2 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Target Information

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that form an important part of the innate immune system.
Alpha-defensins differ from beta-defensins by the pairing of their three disulfide bonds.
Six human beta-defensins (BD-1 to BD-6) have been identified and are expressed on some leukocytes and epithelial surfaces.
In addition to antimicrobial activity, they act as chemoattractants for immature dendritic cells and memory T cells.
Beta-defensins are expressed as C-terminal portions of precursors and released by proteolytic cleavage.
They contain a six-cysteine motif forming three intramolecular disulfide bonds.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.