Thermo Fisher Scientific beta-2 Defensin Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

View 6 publications 6 publications

In vitro Assay (IV)

-

View 1 publication 1 publication

Immunostaining (IS)

-

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human BD-2 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:beta-2 Defensin,uniProtId:O15263-1,ncbiNodeId:9606,antigenRange:24-64,antigenLength:64,antigenImageFileName:500-P161G-100UG_beta-2_Defensin_O15263-1_Goat.svg,antigenImageFileNamePDP:500-P161G-100UG_beta-2_Defensin_O15263-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929556

Product Specific Information

AA Sequence of recombinant protein: GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Human BD-2. Anti-Human BD-2-specific antibody was purified by affinity chromatography employing an immobilized Human BD-2 matrix.

Sandwich ELISA: To detect Human BD-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BD-2 (500-P161GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BD-2.

Western Blot: To detect Human BD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BD-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, six human beta-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors, and are released by proteolytic cleavage of a signal sequence and, in some cases, a propeptide sequence. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.