Thermo Fisher Scientific beta-2 Defensin Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific beta-2 Defensin Polyclonal Antibody, Biotin (PeproTech)

Applications and Tested Dilution

• Western Blot (WB): 0.1–0.2 µg/mL
• ELISA: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human BD-2. Anti-Human BD-2-specific antibody was purified by affinity chromatography and subsequently biotinylated.

Sandwich ELISA:
To detect hBD-2 by sandwich ELISA (using 100 µL/well antibody solution), use a concentration of 0.25–1.0 µg/mL. This biotinylated polyclonal antibody, together with PeproTech Polyclonal Anti-Human BD-2 (500-P161G) as a capture antibody, enables detection of at least 0.2–0.4 ng/well of Recombinant hBD-2.

Western Blot:
To detect hBD-2 by Western Blot, use at 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant hBD-2 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Package Information:
500-P161GBT-1MG is supplied as 2 × 500 µg.

Target Information

Defensins (alpha and beta) are cationic peptides with broad-spectrum antimicrobial activity and form an essential part of the innate immune system.
Alpha-defensins differ from beta-defensins by the pairing of their three disulfide bonds. Six human beta-defensins are known: BD-1, BD-2, BD-3, BD-4, BD-5, and BD-6.
Beta-defensins are expressed on certain leukocytes and epithelial surfaces. Beyond antimicrobial activity, they act as chemoattractants for immature dendritic cells and memory T cells.
These proteins are expressed as C-terminal portions of precursors and released via proteolytic cleavage. Beta-defensins contain a six-cysteine motif forming three intra-molecular disulfide bonds.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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