Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, TRITC
TRITC로 표지된 Donkey anti-Rat IgG (H+L) 항체로, 면역형광(ICC/IF)에 적합합니다. Rat IgG에 특이적으로 반응하며 다양한 종 단백질에 대해 교차 반응이 최소화되어 있습니다. 동결건조 형태로 제공되며, 높은 신호 대비와 안정성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host/Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | TRITC |
| Excitation/Emission Max | 552 / 578 nm |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | Store at 4°C (short term). For long term, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535527 |
| Target | IgG |
| Cross Adsorption | Bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, sheep IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
- Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve.
- Product is overfilled to ensure complete recovery.
- Centrifuge to remove particulates.
- Prepare fresh working dilution daily.
- Stable for 1 year from date of receipt.
- Based on immunoelectrophoresis, no reactivity to non-immunoglobulin rat serum proteins or IgG from other species listed above.
Target Information
Anti-Rat secondary antibodies are affinity-purified and show high specificity for rat immunoglobulins.
They are used for detection, sorting, or purification of rat targets.
Secondary antibodies allow flexible detection systems (e.g., HRP, AP, fluorescence) and enhanced sensitivity via signal amplification.
Typically, these antibodies are generated by immunizing the host with pooled immunoglobulins from the target species and further purified or conjugated for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 647
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- AP
- Biotin
- FITC
- HRP
- Custom conjugation available
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