Thermo Fisher Scientific PAI1 Monoclonal Antibody (MA-33H1F7)

Thermo Fisher Scientific PAI1 Monoclonal Antibody (MA-33H1F7)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

MA1-40224 detects PAI-1 from human, rat, mouse, and rabbit samples.
It has been successfully used in ELISA, functional studies, and Western blot applications.
The immunogen is human PAI-1, and endotoxin level is <24 EU/mg.

The epitope (hF epitope) of MA1-40224 consists of three residues (Lys154, Glu130, Arg131) positioned linearly in the 3D structure.
This epitope does not cover the complete alpha-helix F but is restricted to the hinge region between alpha-helix F and the main part of PAI-1.
MA1-40224 is a ‘switching’ antibody capable of inducing a non-inhibitory substrate form of PAI-1 and inhibits PAI-1 in a dose-dependent manner.

Target Information

PAI1 (plasminogen activator inhibitor 1) is a member of the serine protease inhibitor superfamily and the principal inhibitor of tissue-type and urokinase-type plasminogen activators (tPA and uPA).
Platelets are the main source of circulating PAI1, but it is also synthesized by fibroblasts, smooth muscle cells, endothelial cells, hepatocytes, and inflammatory cells.

Expression of PAI1 is regulated by various factors including growth factors, cytokines, hormones, inflammatory factors, metabolites, and environmental influences.
Elevated levels are associated with thrombosis, obesity, thrombophilia, and metabolic syndrome.
Defects in PAI1 cause abnormal bleeding and are linked to plasminogen activator inhibitor-1 deficiency.
PAI1 inhibits fibrinolysis by blocking plasminogen activator activity and may promote neuronal survival.
Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.