Thermo Fisher Scientific IL-6 Monoclonal Antibody (MP5-20F3), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 5 publications 5 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

Flow Cytometry (Flow)

-

View 16 publications 16 publications

ELISA (ELISA)

-

View 23 publications 23 publications

Neutralization (Neu)

-

View 6 publications 6 publications

Functional Assay (Functional)

-

View 3 publications 3 publications

Blocking Assay (BLOCK)

-

View 1 publication 1 publication

Immunostaining (IS)

0.5-2 µg/mL

-

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

MP5-20F3

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Brilliant Violet 650
• eFluor 450
• FITC
• Functional Grade
• PE
• PerCP-eFluor 710
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468422

Product Specific Information

Description: The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody is a neutralizing antibody. mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.

Applications Reported: This MP5-20F3 antibody has been reported for use in ELISA capture antibody and ELISPOT capture antibody.

Applications Tested: The MP5-20F3 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-6 (IL-6) in combination with the biotin MP5-32C11 (Product # 13-7062-81) antibody for detection and recombinant mouse IL-6 (Product # 14-8061-80) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 8-1000 pg/mL should be included in each ELISA plate.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.