Thermo Fisher Scientific SPG7 Monoclonal Antibody (OTI5H7), TrueMAB
Human SPG7 단백질을 인식하는 Mouse monoclonal antibody로 Western blot 및 Immunocytochemistry에 사용 가능. Lyophilized 형태로 제공되며, PBS buffer에 trehalose 포함. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5H7 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 300–573 of human SPG7 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended desalting product: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
The SPG7 gene encodes a mitochondrial metalloprotease belonging to the AAA protein family.
Members of this family share an ATPase domain and participate in various cellular processes such as:
- Membrane trafficking
- Intracellular motility
- Organelle biogenesis
- Protein folding
- Proteolysis
Mutations in SPG7 cause autosomal recessive spastic paraplegia 7.
Two distinct transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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