Thermo Fisher Scientific SPG7 Monoclonal Antibody (OTI3E8), TrueMAB
SPG7 단백질을 인식하는 mouse IgG1 단일클론 항체로 Western blot 및 Immunocytochemistry에 적합. 인간 시료 반응성, 무보존제 제형, 동결건조 상태로 제공. 연구용으로 사용되며 재구성 및 결합 실험에 용이.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3E8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 300–573 of human SPG7 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The SPG7 gene encodes a mitochondrial metalloprotease protein, a member of the AAA family. These proteins contain an ATPase domain and participate in various cellular processes such as:
- Membrane trafficking
- Intracellular motility
- Organelle biogenesis
- Protein folding
- Proteolysis
Mutations in this gene are associated with autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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