Thermo Fisher Scientific ZSCAN4 Monoclonal Antibody (OTI5E8), TrueMAB
인간 ZSCAN4 단백질(aa 164-433)에 특이적인 마우스 단클론 항체. Western blot에 최적화된 TrueMAB 형식. 동결건조 형태로 제공되며, 재조합 후 1 mg/mL 농도 유지. 줄기세포 및 배아 발달 연구용으로 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5E8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 164–433 of human ZSCAN4 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Zygotic genome activation (ZGA) marks the transition from maternal to embryonic genetic control, with a major burst occurring at the late 2-cell stage.
ZSCAN4 encodes a SCAN domain and four zinc finger domains, expressed in late 2-cell embryos and embryonic stem cells.
Loss-of-function studies using siRNA show delays in progression from the 2-cell to 4-cell stage and subsequent embryo development.
ZSCAN4 is essential for preimplantation development, telomere maintenance, and long-term genomic stability in embryonic stem cells, suggesting its critical role in maintaining pluripotency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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