Thermo Fisher Scientific CD51/CD61 Monoclonal Antibody (23C6)
CD51/CD61 단백질 복합체를 인식하는 Mouse IgG1 단일클론 항체로, 인체 및 조류 시료에 반응함. IHC, ICC, Flow Cytometry, IP 등 다양한 응용 가능. 포르말데하이드 민감성을 가지며 동결 절편에는 아세톤 고정 권장. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | View 1 publication |
| Flow Cytometry (Flow) | 1:40–1:80 | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chicken, Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 23C6 |
| Immunogen | Osteoclasts from osteoclastomas |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537984 |
Product Specific Information
- Does not react with bovine, pig, or rabbit.
- Epitope is sensitive to formaldehyde fixation and tissue processing; acetone fixation recommended for frozen sections.
- For FACS analysis, use 10 µL of the suggested working dilution to label 1×10^6 cells in 100 µL.
Target Information
CD51 and CD61 molecules (alpha V and beta 3 integrins) form a complex that binds vitronectin at the RGD sequence.
This complex can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin, and collagen.
Reacts with osteoclasts, placenta, melanoma cell lines, and weakly with platelets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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