Thermo Fisher Scientific Rubicon Polyclonal Antibody
Rubicon 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, Western Blot 및 Immunohistochemistry에 사용 가능. 인간, 마우스, 랫트 반응성. 합성 펩타이드 항원 기반으로 제작되었으며, 리오필라이즈드 형태로 제공. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200–1:2,000 | - |
| Immunohistochemistry (IHC) | 1:200–1:2,000 | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from aa region 750–800 of mouse Rubicon conjugated to an immunogenic carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
- The peptide is homologous in human and rat.
- Specificity: Rubicon.
Target Information
Rubicon (RUN domain Beclin-1 interacting and cysteine-rich containing protein) and ATG14L are Beclin-1-interacting proteins that reciprocally regulate autophagy at different stages.
Knockdown of Rubicon enhances autophagy, while knockdown of ATG14L causes a defect in autophagosome formation.
Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and serves as a positive regulator of the NADPH oxidase complex.
Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NADPH oxidase complex, leading to reactive oxygen species production and inflammatory cytokine release.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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