Thermo Fisher Scientific Tubulin beta 3/TUBB3 Monoclonal Antibody (TUBB3, 3732)
신경세포 기원의 표지자로 사용되는 Tubulin beta 3 단백질을 인식하는 단일클론 항체. Western blot, IHC, Peptide array 등 다양한 응용 가능. 고순도 Protein A/G 정제, 액상 형태로 제공. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: Assay-dependent
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Peptide Array (Array)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TUBB3, 3732 |
| Immunogen | A synthetic peptide (aa 437–450) of human Tubulin beta 3 protein (TUBB3); coupled to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
The betaIII-tubulin isoform is dominantly present in cells of neuronal origin and is one of the earliest markers of neuronal differentiation.
It serves as a specific probe for neuronal cells and tumors derived from them.
BetaIII-tubulin is most abundant in neuronal cells but is also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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