Thermo Fisher Scientific Interferon regulatory factor 9/IRF-9 Monoclonal Antibody (PCRP-IRF9-2F8)
인간 IRF9 단백질을 인식하는 마우스 모노클로날 항체로, ICC/IF, Flow Cytometry, Peptide Array 등에 사용 가능. 항바이러스 면역 신호전달 연구에 적합하며, IFN-alpha/beta 활성화 후 핵으로 이동하는 IRF9 검출에 유용. 단백질 A/G로 정제된 액상형 항체.
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Thermo Fisher Scientific Interferon Regulatory Factor 9 (IRF-9) Monoclonal Antibody (PCRP-IRF9-2F8)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PCRP-IRF9-2F8 |
| Immunogen | Recombinant full-length human IRF9 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Antibody is stable for 24 months.
- Positive Control: MCF-7, Jurkat, or K562 cells
- Cellular Localization: Cytoplasm and nucleus; translocated into the nucleus upon activation by IFN-alpha/beta
- Specificity Comments: Transcription regulatory factor mediating signaling by type I interferons (IFN-alpha and IFN-beta). Upon IFN binding, TYK2 and JAK1 kinases phosphorylate STAT1 and STAT2, which dimerize and associate with IRF9 (ISGF3G) to form the ISGF3 transcription factor complex that enters the nucleus and binds to the IFN-stimulated response element (ISRE), activating transcription of antiviral genes.
Target Information
Transcription factor essential for antiviral immunity. IRF9 mediates type I IFN signaling via association with phosphorylated STAT1:STAT2 dimers to form the ISGF3 complex. ISGF3 binds ISRE sequences to activate transcription of interferon-stimulated genes, driving antiviral cellular responses. [UniProt]
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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